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Methodes chromatographiques

3CAHC1 Methodes chromatographiques Materials and Chemistry S9
Lessons : 15 h TD : 0 h TP : 0 h Project : 0 h Total : 15 h
Co-ordinator : Pascal Cardinael
Prerequisite
Fundamental quantities in chromatography

The basics of GC and CPG

Works:

-Chemical analysis methods and instrumental techniques, Francis Rouessac, Annick Rouessac, Daniel Cruché, et al., Collection: Sciences Sup, Dunod 2009-7th edition

-Analytical chemistry, Douglas A. Skoog, Stanley R. Crouch, F. James Holler, Donald M. West

Translation: Claudine Buess-Herman, Josette Danner 2012 Editor: De Boeck
Course Objectives
Acquire the necessary tools to develop the analysis of all types of organic compounds in HPLC and introduce students to the latest innovations in separative methods
Syllabus
1-Optimization of the analysis

2 – Analysis of polar and ionizable compounds (choice of stationary phase, mobile phase...)

3 – High temperature liquid chromatography (HTLC) and ultra high pressure chromatography (U-HPLC) practical advantages and constraints

4-Theoretical and practical Aspects of miniaturization

5 – Detection and coupling

6 – Analysis of chiral compounds by GC and HPLC

7 – Two-dimensional and multidimensional chromatography
Practical work (TD or TP)
Non renseigné
Acquired skills
-Control of the development of an analysis in HPLC including the choice of the column and the operating parameters.

-Control of multidimensional gas chromatography methods.
Bibliography
- Chromatographies en phases liquide et super critique Rosset R., Caude M. et Jardy A. 1996 - 3è éd., Masson

- Introduction to modern liquid chromatography (3rd Ed.)Auteurs : Snyder Lloyd R., Kirkland Joseph J., Dolan John W.Wiley 2010

- Chromatographic enantioseparation: Methods and applications, S. G. Allenmark. (1988). Ellis Horwood, Chichester

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